급성 비부비동염이 유발된 생쥐에서 항생제의 국소 투여 효과

Other Titles
Effect of topical antibiotics to mouse model of acute rhinosinusitis
Authors
송인혁
Issue Date
2006-12
Awarded Date
2007
Abstract
급성 비부비동염의 치료에 있어 항생제 투여는 필수적이다. 비강내 항생제의 국소투여는 약제의 생체 이용율에 있어 제한적일 수 있으나 매우 효과적인 치료법의 하나로 생각된다. 이에 급성 비부비동염의 원인균 중 가장 빈도가 높은 폐렴연쇄구균을 생쥐에 국소투여하여 비부비동염을 유발시킨 후, 시간의존성 항생제와 농도의존성 항생제를 국소투여하여 그 효과를 알아보고자 하였다. 생후 10 주의 23-27 g의 C57BL/6 수컷 생쥐 50 마리를 이용하여 음성대조군을 제외한 모든 생쥐에게 폐렴연쇄구균을 접종하여 급성 비부비동염을 유발시킨 후 6일째부터 하루 1 회 5 일간 생리식염수만 접종한 음성대조군(I군),시간의존성 항생제군(cefmenoxime and vancomycin, II군)와 농도의존성 항생제군(ofloxacin and tobramycin, III군), 양성대조군(IV군)으로 분류하였고 11일째 비부비강을 세척한 액을 배양시키고, 비부비강 조직을 H&E 염색하여 중성구의 숫자를 비교하였다. 폐렴연쇄구균에 대한 각 항생제의 최소살균농도(MIC)는 cefmenoxime 20 μg/mL, ofloxacin 80 μg/mL, tobramycin 25 μg/mL, vancomycin 12.5 μg/mL이었다. 균주배양검사상 항생제 투여군인 II, III군은 양성대조군보다 감소된 소견을 보였으며 이는 통계학적 의의가 있었다(p=0.037). 농도의존성 항생제와 시간의존성 항생제, 두 군을 비교할 때 농도의존성 항생제군이 시간의존성 항생제군보다 중성구군집이 더 적었으며 이는 통계학적 의의가 있었다(p=0.001). 폐렴연쇄구균으로 유발된 급성비부비동염의 치료로서 농도의존성항생제의 국소투여가 시간의존성항생제보다 효과적임을 알 수 있었다.
Administration of antibiotics is essential modality to treat acute rhinosinusitis. Although intranasal inoculation of antibiotics does not have definite bioavailability, it is very effective method to treat acute rhinosinusitis. This study was performed a mouse model of rhinosinusitis by inoculation of Streptococcus pneumoniae and administrated time-dependent antibiotics or concentration-dependent antibiotics as a topical manner and investigated their effectiveness. Fifty ten-week old male C57BL/6 mice were employed for acute rhinosinusitis model by inoculation of Streptococcus pneumoniae except negative control. From 6th day to 10th day we made negative control group by inoculation of normal saline (Group I), antibiotics group by inoculation of cefmenoxime and vancomycin (Group II), ofloxacin and tobramycin (Group III), and positive control group (Group IV). On the 11th day, all mice were sacrificed and the effectiveness of antibiotics was estimated by comparison of nasal lavage colony count and neutrophil count of sinonasal tissue. Minimal bactericidal concentration of each antibiotics was cefmenoxime 20 μg/mL, ofloxacin 80 μg/mL, tobramycin 25 μg/mL, vancomycin 12.5 μg/mL. By nasal lavage, antibiotics inoculation group (Group Ⅱ, Ⅲ) showed decreased bacterial growth than positive control significantly (p=0.037). In comparison between the group administrated with concentration- dependent antibiotics and time-dependent antibiotics, clusters of neutrophil decreased in two groups compared to positive control, the group administrated with concentration-dependent antibiotics had fewer clusters of neutrophil than the group administrated with time-dependent antibiotics, and it was statistically significant (p=0.001). Local inoculation of concentration-dependent antibiotics could be more effective way to treat acute rhinosinusitis induced by Streptococcus pneumoniae than time-dependent antibiotics.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/11843
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3. Thesis (학위논문) > 1. School of Medicine (의과대학) > 석사
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