흰쥐 담즙울체간에서의 알콜대사 효소들의 활성치

Abstract

Changes in the activities of the following alcohol metabolizing enzymes have been studied over a period of forty two days after the ligation of common bile duct in rats: cytosolic alcohol dehydrogenase (ADH) microsomal ethanol oxidizing system (MEOS) and catalase of cholestatic liver. The activities of aldehyde dehydrogenase (ALDH) in the cytosolic, microsomal and mitochondrial fractions of cholestatic liver were also measured. The effect of common dile duct ligation on the liver ADH, MEOS , catalase and ALDH activities in ethanol ingested rats were studied. The animals were given 5%(v/v)ethanol solution for 74 days and these were ligated of common bile duct at 2 weeks prior to killings. The cyctosolic ADH activities in the cholestatic liver decreased significantly between the seventh and forty-second day after the ligation of common bile duct in rats However, MEOS activities in cholestatic liver showed a substantial increase from three days to forty two days after the operation. The catalase activities in the cholestatic rat liver decreased significantly between third and forty-second day after the operation. The activities of cytosolic and microsomal ALDH in the cholestatic liver increase drastically between the third and forty-second days after the operation, but the mitochondrial ALDH in the cholestatic liver had a significant diminution from twenty weight days to forty two days after the ligation of common bile duct. The liver cytosolic ADH activities decreased in rats given ethanol with additional common bile duct ligation treatment (ethanol 74days-CBDL 2 weeks group). However, the activities showed a far more degree than group of common bile duct ligation treatment at 2 weeks prior to killings (CBDL 2 weeks group). The liver MEOS activities increased in rats given ethanol for 74 days (ethanol 74 days group) and ethanol 74 days-CBDL 2 weeks group. But the liver MEOS activities of ethanol 74 days-CBDL 2 weeks group remained within the limit of CLDL 2weeks group. The liver catalse activities decreased in ethanol 74 days -CBDL 2 weeks group. The activities were not significantly affected by common bile duct ligation. The liver microsomal ALDH activities increased markedly in ethanol 74 days group and ethanol 74 days-CBDL 2 weeks group but cytosolic and mitochondrial ALDH activities did not increase in these groups.