인체편평상피세포의 세포유전학적 분석

Abstract

Cytogenetic analysis was performed in human squamous cell carcinoma untreated with drugs or radiation. Tumor was disaggregated with mechanical and enzymatic methods. Long-term trypsinization(4'c, 24 hours) reproduced more cell yields than short-term trypsinization(37'c, 30-60 minutes) however, the frequency of metaphases was no significance. Disaggregated cells were treated with colcemid(5ug/ml) for 3 hours. Recognizable metaphase chromosomes could be obtained from conventional preparation of chromsomes. The results were summarized as follows: Chromosomal range was 42 to 50, but one cell line showed near-tetraploid. There were no findings of specific chromosomal abnormalties such as structural rearrangement or marker chromsome. Nonrandom chromosomal losses were found at chromosome #10, #12, #15, #17, #22. However, these chromosomal losses are of no significance since these cells will die soon. Specific findings in this study were trisomy #1, #7. It is known that ras oncogens is located in the chromosome #1 and raf-2 oncogene in the chromosome #7. Further study is in need to clarify the relation between these oncogenes and trisomy in squamous cell carcinoma.