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인체편평상피세포의 세포유전학적 분석

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Author(s)
편영식
Alternative Author(s)
Pyun, Young Sik
Publication Year
1989
Abstract
Cytogenetic analysis was performed in human squamous cell carcinoma untreated with drugs or radiation. Tumor was disaggregated with mechanical and enzymatic methods. Long-term trypsinization(4'c, 24 hours) reproduced more cell yields than short-term trypsinization(37'c, 30-60 minutes) however, the frequency of metaphases was no significance. Disaggregated cells were treated with colcemid(5ug/ml) for 3 hours. Recognizable metaphase chromosomes could be obtained from conventional preparation of chromsomes. The results were summarized as follows: Chromosomal range was 42 to 50, but one cell line showed near-tetraploid. There were no findings of specific chromosomal abnormalties such as structural rearrangement or marker chromsome. Nonrandom chromosomal losses were found at chromosome #10, #12, #15, #17, #22. However, these chromosomal losses are of no significance since these cells will die soon. Specific findings in this study were trisomy #1, #7. It is known that ras oncogens is located in the chromosome #1 and raf-2 oncogene in the chromosome #7. Further study is in need to clarify the relation between these oncogenes and trisomy in squamous cell carcinoma.
Alternative Title
Cytogenetic Analysis on Primary Squamous Cell Carcinoma in Human
Department
Dept. of Orthopedic Surgery (정형외과학)
Publisher
Keimyung University School of Medicine
Citation
Keimyung Medical Journal, Vol.8(1) : 54-60, 1989
Type
Article
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/14560
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