주정 중독 흰쥐 간의 Glyoxalase Ⅱ 활성에 미치는 총담관 결찰의 영향

Abstract

To understand the biochemical background of alcohol hazards in hepatobiliary disease, the activities of the rat liver cytosolic and mitochondrial glyoxalase-Ⅱ(GLO-Ⅱ) were measured in cholestasis induced by common bile duct (CBD) ligation after chronic ethanol intoxication, and in cholestasis before acute ethanol intoxication. GLO-Ⅱ activities in the rat's liver cytosol and mitochondria showed more decrease when CBD was ligated after chronic ethanol intoxication than that in the CBD ligation alone. On the other hand, when CBD was ligated after chronic ethanol intoxication, the value of Vmax of the liver cytosolic and mitochondrial GLO-Ⅱ decreased significantly than that in the CBD was ligated alone. However, the values of Km of the liver cytosolic and mitochondrial GLO-Ⅱ did not change in the all experimental groups. Viewed from above results, when chronic ethanol intoxication is combined with cholestasis, GLO-Ⅱ in the liver seems to be decreased its activity than that in cholestasis, and the cause of the decrease is thought to be decreased biosynthesis. Accordingly, this result will be the datum supporting that alcoholic drink to be enzymologically harmful in hepatobiliary disease.