흰쥐 담즙울체간에서의 Glucose-6-Phosphate Isomerase 및 Fructose-1, 6-Bisphosphate Aldolase의 활성도

Abstract

This study was made to know the changes of glucose-6-phosphate isomerase (GPI) and fructose-1, 6-bisphosphate aldolase (ALS) activities in cholestatic rat livers. GPI and ALS activities were determined in cytosolic, mitochondrial, and microsomal preparations isolater from cholestatic rat liver, and in the rat serum after common bile duct ligation for a period of forty two days. The activities of mitochondrial and microsomal GPI in cholestatic rat liver showed a significant increase from the second day to the forty second day, and from the first day to the twenty eighth day, respectively, after the ligation. However, the cytosolic GPI activity in cholestatic liver showed a significant decrease from the twelfth hour to the second day after the ligation. The activities of both mitochondrial and microsomal ALS in cholestatic rat liver showed a significant increase from the second day to the third day after the ligation. However, cytosolic ALS in cholestatic liver was decreased slightly from the first day to the forty second day after the ligation. On the other hand, the activities of GPI and ALS in rat serum showed a significant increase from the twelfth hour to the seventh day, and from the first day to the forty second day, respectively, after the ligation. These results, therefore, suggest that the biosynthesis of liver particulate-bound GPI and ALS are induced in response to cholestasis. And the elevations of both serum GPI and ALS activities seem to be leak of the enzymes into the blood from cholestatic liver