개회충 성충항원을 이용한 실험적 토끼 개회충증 혈청 항체가의 측정

Abstract

Toxocariasis is produced by the migration of the Toxocara canis larvae into the extra-intestinal tissues of unnatural hosts or natural hosts under suitable conditions. Generally, serodiagnosis and treatment may be difficult in this situation. The present study was performed to observe the specifically reacting antigenic bands in crude extract of Toxocara canis adult worm and its reaction in the serum IgG antibody obtained from experimentally infected rabbits. After homogenization, the homogenate was extracted at 4℃ for 2 days and centrifuged at 20,000 x g for 1 hour. The supernatant was used as the antigen. Infected sera were obtained from the rabbits infected with Toxocara canis embryonated eggs. The control sera were obtained from non-infected rabbits. The ELISA showed that serum levels of IgG antibody against crude antigen began to increase from 10 to 14 days after the infection. SDS-PAGE profile showed complex protein bands ranging 6.5 to 200 kDa. Of them 110, 62.5, 48, 17.5, and 13.5 kDa components were prominent bands. according to the immunoblot data, the serum antibody recognized major protein bands with molecular weight of 110, 101, 72, 62.5, 48, 42, 32.5, and 13.5 kDa respectively. The 110, 72,62.5, and 48 kDa bands reacted even in non-infected control sera. Cross reactions by immunoblot were also observed with the sera from infected and immunized with Trichinella and Anisakis. The proteins of 110 and 42 kDa showed as common antigens. In conclusion, Toxocara canis adult worm antigen represents an antigen that can be used in the diagnosis of toxocariasis. And the 101, 32.5, and 13.5 kDa band were specific antigenic proteins.