상온보관이 가능하며 사용이 편리한 DNA Size-Marker와 저분자량의 DNA 계측을 위한 Size Marker용 플라스미드 개발

Other Titles
Developments of Ready-Made DNA Size Markers and a Novel Plasmid for a Low Molecular Weight Size Marker.
Authors
박종구
Department
Dept. of Molecular Medicine (분자의학); Institute for Medical Science (의과학 연구소)
Issue Date
1998
Citation
Keimyung Medical Journal, Vol.17(4) : 535-541, 1998
Abstract
Molecular biotechnology is a broad research and development principle encompassing basic research as well as applied industrial developments. Biotechnology is expected to become one of the most important research and development sectors in the next century, but the domestic support industry for the field is severely underdeveloped and at best in its infancy. DNA size marker is frequently employed during research in a purpose of extrapolating the relative size of a DNA fragment or measuring the amount of DNA loaded a sample. However, DNA size marker is not developed for commercialization domestically. Further, liquid suspension containing DNA size marker needs to be kept at a low temperature during storage and shipping. In an attempt to maintain the DNA samples at room temperature for extended period of time, lyophilization of DNA with addition of a nuclease inhibitor was studied. Hae Ⅲ digest of ΦX174 phage DNA has been a useful size marker for low molecualr weight DNA molecules, but the double strand RF form of the phage DNA requires laborious procedures for cell culture and purification. A new plasmid was constructed to generate band patterns which can substitute the Hae Ⅲ digest of ΦX174 phage DNA for a comparable purpose. Gel staining dye was also added to the lyophilized DNA to provide additional convenience such that DNA size marker was ready for use by simple reconstitution of distilled water. DNA size marker appears to be advantageous for development as one can judge the quality of the product by direct observation.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/15113
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
2. Keimyung Medical Journal (계명의대 학술지) > 1998
Keimyung Author(s)
박종구
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