흰쥐 재생간에서 Benzoyltransferase 및 Phenylacetyltransferase의 활성도

Abstract

We have investigated the effect of partial hepatectomy on the closely related acyl-CoA : amino acid N-acyltransferase, benzoyltransferase and phenylacetyltransferase activities in rat liver. Cytosolic, mitochondrial and microsomal benzoyltransferase and phenylacetyltransferase activities were determined in regenerating livers after 70% (median and left lateral lobes) partial hepatectomy in rats over a pariod of ten days. The activities of both benzoyltransferase and phenylacetyltransferase in microsome of regenerating rat liver showed a significant increase compared to the activities from the original liver from the first day to the third day after partial hepatectomy. The enzyme kinetic parameters of hepatic benzoyltransferase were analyzed using benzoyl coenzyme A as a substrate with microsomal preparations from the first day post-hepatectomy. And enzyme parameters of hepatic phenylacetyltransferase were also analyzed using phenylacetyl coenzyme A as a substrate with microsomal preparations from the first day post-hepatectomy. The results indicated that although the Km values of these enzymes were about the same as the control original liver, the Vmax values of both enzymes increased significantly. These results, therefore, suggest that the biosynthesis of hepatic microsomal benzoyltransferase and phenylacetyltransferase has been increased in the regenerating stage.