MAGE isotypes 또는 GAGE isotypes의 DNA 및 단백질 동질성

Abstract

Melanoma antigens genes(MAGE) and GAGE are cancer-associated antigens. Because these antigens are expressed in several types of cancer and recognized by cytotoxic T cell, they have been used as cancer diagnosis markers or antigens for induction of anticancer immunity. Here, we studied DNA and protein homology between MAGE isotypes or GAGE isotypes to evaluate their homology can be used for cancer diagnosis or cancer immunotherapy. DNA homology of MAGE family was between 99% and 56%, and protein homology of MAGE family was between 96% and 51%. Comparisons between MAGE-3 and MAGE-6 showed the highest DNA and protein homology. DNA homology of GAGE family was between 99.6% and 82%, and protein homology of GAGE family was between 99% and 93%. All of these results suggest that DNA homology of MAGE or GAGE family can be used for designing primer that can amplify multiple isotypes together, and protein homology of GAGE family may be used for designing cancer epitope that induce cross reactive immune response.