피부 각질화세포에서 자외선 B 조사로 발현이 유도된 유전자의 검출

Abstract

Ultraviolet light is the most important environmental insult to skin. Even single exposure to UVB irradiation can result in inflammation and may also lead to DNA damage and apoptosis in the acute response of the cutaneous tissue. To elucidate the complex alterations of gene expression in human keratinocytes underlying these UVB responses, ordered differential display polymerase chain reaction (ODD-PCR) technology was performed in one human keratinocyte cell line. Several genes were induced and down-regulated during 12 hours after UVB irradiation in a time- dependent manner. Five UVB-induced cDNA clones were isolated, including cDNAs for karyopherin alpha 2, human mRNA for TI-227H, ribosomal protein L13a and keratin 19. Differential expression of these genes after UVB irradiation has not been reported. Authors identified a new sequence that is negatively regulated by UVB irradiation. In general our results showed that ODD- PCR is a useful tool in the analysis of quantitative changes of mRNA levels in human keratinocytes after UVB irradiation. The identification of new UVB-modulated genes offers the opportunity to identify unrecognized molecular mechanism in response of human cells to UV irradiation.