만성 주정 중독 쥐에서 총담관 결찰이 간의 Aryl Sulfotransferase Ⅲ,Ⅳ 의 활성에 미치는 영향

Abstract

The drinking hazard in hepatobiliary disease was studied by the assays of hepatic aryl sulfotransferase Ⅲ, Ⅳ isozyme activies in cholestasis induced by common bile duct (CBD) liga­tion after chronic ethanol intoxication in rats. The activity of serum aryl sulfotransferase Ⅲ, Ⅳ isozyme was also measured. Liver cytosolic aryl sulfotransferase Ⅲ, Ⅳ isozyme activity in the group of CBD ligation after chronic ethanol intoxication showed a greater decrease between the 7th and the 14th day after CBD ligation than that in the group of CBD ligation alone. The mitochondrial aryl sul­fotransferase Ⅲ, Ⅳ isozyme activity in the group of CBD ligation after chronic ethanol intoxication showed a greater decrease at the 14th day after CBD ligation than that in the group of CBD ligation alone. The microsomal arylsulfotransferase Ⅲ, Ⅳ isozyme activity in the CBD ligation after chronic ethanol intoxication showed a greater decrease between the 2nd and 14th day after CBD ligation than that in the group of CBD ligation alone. The serum aryl sulfotrnasferase Ⅲ, Ⅳ isozyme activity in the CBD ligation after chronic ethanol intoxication showed a greater increase between the 1st and 14th day after CBD ligation than that in the group of CBD ligation alone. The Vmax values of the liver cytosolic and mitochondrial aryl sulfotransferase Ⅲ, Ⅳ isozyme in the CBD ligation after chronic ethanol intoxication showed a greater decrease at the 14th day after CBD ligation than that in the group of CBD ligation alone. However, the Km values of the above hepatic enzymes did not change. The results indicate that the biosynthesis of the liver aryl sulfotransferase Ⅲ, Ⅳ isozyme is decreas­ing in chronic ethanol intoxication with cholestasis than in cholestasis alone. The increased activity of the serum aryl sulfotransferase Ⅲ, Ⅳ isozyme in chronic ethanol intoxication with cholestasis reflects increased hepatic damages which cause this isozyme to leak into the blood in great quantity.