담즙울체 쥐에서 간 라이소솜의 α-D-Glucosidase와 β-D-Glucuronidase 활성도에 미치는 Taurocholate의 정맥 내 투여의 영향

Abstract

The effects of intravenously administered of high concentration of taurocholic acid (TCA) on α-D-glucosidase and β-D-glucuronidase activities in rat liver lysosomes were studied. These liver lysosomal enzymes, and serum lysosomal α-D-glucosidase and total β-D-glucuronidase activities were also determined in experimental rats with common bile duct ligation (CBDL). The activities of liver lysosomal α-D-glucosidase and β-D-glucuronidase as well as the activities of serum α-D-glucosidase and total β-D-glucuronidase were found to be significantly increased in the CBDL plus TCA injected group than in the control group such as CBDL alone group. On the other hand, these serum and hepatic enzymes activities did not change in the CBDL plus tauroursodeoxycholic acid injected group. The above results suggest that TCA induces biosynthesis of the lysosomal α-D-glucosidase and β-D-glucuronidase in the liver and that the elevated lysosomal α-D -glucosidase and total β-D-glucuronidase activities in the serum are most likely due to increased hepatocyte membrane permeability caused by TCA mediated liver cell necrosis.