총담관을 결찰한 쥐에서 간의 Phenylacetyltransferase 활성도에 미치는 Taurocholate의 정맥 내 투여의 영향

Abstract

The effect of intravenous administeration of high dose taurocholate on phenylacetyltransferase (PAT) activity was studied in rat liver. PAT activity, and it’s Km and Vmax values were determined in the liver cytosolic, mitochondrial and microsomal fractions isolated from the common bile duct (CBD) ligated rats, which were injected with taurocholic acid (TCA), The activities of liver cytosolic, mitochondrial and microsomal PAT as well as their Vmax values were found to be significantly increased in the CBD ligation plus TCA injected group than in the control group, such as CBD ligation alone group. However, their Km values in the experimental group did not vary. On the other hand, these hepatic enzyme activities did not change in the CBD ligation plus tauroursodeoxycholic acid injected group. Therefore, the above results indicate that TCA stimulates biosynthesis of the PAT in the liver