만성 주정중독 쥐에서 간의 Rhodanese 활성도에 미치는 간외성 담즙울체의 영향

Abstract

To investigate the adverse effects of alcohol ingestion on the liver of hepatobiliary disease, the activity of rat serum and hepatic rhodanese was measured in extrahepatic cholestasis induced by common bile duct (CBD) ligation after chronic ethanol intoxication. Liver cytosolic, mitochondrial and microsomal rhodanese activities in CBD ligated rats with chronic ethanol intoxication were found to be decreased much more than that in CBD ligation alone. Vmax values of the liver cytosolic and mitochondrial rhodanese in these animals were lower than in the control, whereas Km values of the above hepatic enzymes did not vary in the all experimental groups. On the other hand, serum rhodanese activity in CBD ligated rats with chronic ethanol intoxication was greater increased more than that in CBD ligation alone. These results indicate that the biosynthesis of the hepatic rhodanese decreases and the serum rhodanese activity increases in cholestasis combined with chronic ethanol intoxication, reflecting damages of aggravated hepatocytic membrane.