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콜라겐 유발성 관절염에 대한 IL-4발현 재조합 아레노바이러스의 효과

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Author(s)
박종구
Alternative Author(s)
Park, Jong Gu
Publication Year
2003
Keyword
Collagen induced arthritisInterleukin-4Recombinant adenovirus
Abstract
Interleukin-4 (IL-4) is one of anti-inflammatory cytokines which has
the potential to counteract pro-inflammatory cytokines often detected in
rheumatoid arthritis. This study examined the effect of adenovirus-mediated gene
transfer of IL-4 on collagen-induced arthritis (CIA), an animal model of
rheumatoid arthritis. The IL-4 cDNA was cloned from a T lymphoma cell line after
induction with PMA and was further cloned into an adenovirus shuttle vector. The
recombinant adenovirus encoding rat IL-4, AdrIL-4, was constructed by
homologous recombination between the cloned shuttle vector and a rescue vector,
pJM17, and identified by polymerase chain reaction. CIA was induced in Dark
agouti (DA) rats by immunizing type II collagen. On 12th day after immunization,
DA rats were administrated with intra-articular injection of 4×108 plaque-forming
units of AdrIL-4. The exogenous IL-4 expression was observed to inhibit CIA
progression, compared to those of control groups, in which PBS or recombinant
adenovirus without a transgene, Adnull, was administered. Whereas any
significant difference was not observed on the production of anti-collagen
antibodies among different groups of rats, IL-4 gene transfer but not with control
treatments was shown to suppress CIA in a moderate manner.
Alternative Title
Effect of IL-4
Expressed by Recombinant Adenovirus
on Collagen Induced Arthritis
Department
Dept. of Molecular Medicine (분자의학)
Publisher
Keimyung University School of Medicine
Citation
Keimyung Medical Journal, Vol.22(1) : 41-49, 2003
Type
Article
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/15264
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