콜라겐 유발성 관절염에 대한 IL-4발현 재조합 아레노바이러스의 효과

Abstract

Interleukin-4 (IL-4) is one of anti-inflammatory cytokines which has the potential to counteract pro-inflammatory cytokines often detected in rheumatoid arthritis. This study examined the effect of adenovirus-mediated gene transfer of IL-4 on collagen-induced arthritis (CIA), an animal model of rheumatoid arthritis. The IL-4 cDNA was cloned from a T lymphoma cell line after induction with PMA and was further cloned into an adenovirus shuttle vector. The recombinant adenovirus encoding rat IL-4, AdrIL-4, was constructed by homologous recombination between the cloned shuttle vector and a rescue vector, pJM17, and identified by polymerase chain reaction. CIA was induced in Dark agouti (DA) rats by immunizing type II collagen. On 12th day after immunization, DA rats were administrated with intra-articular injection of 4×108 plaque-forming units of AdrIL-4. The exogenous IL-4 expression was observed to inhibit CIA progression, compared to those of control groups, in which PBS or recombinant adenovirus without a transgene, Adnull, was administered. Whereas any significant difference was not observed on the production of anti-collagen antibodies among different groups of rats, IL-4 gene transfer but not with control treatments was shown to suppress CIA in a moderate manner.