만성 주정중독 쥐간의 Epoxide Hydratase 활성에 미치는 간외성 담즙울체의 영향

Abstract

To investigate the adverse effects of alcohol ingestion to the liver under the hepatobiliary disease, the activity of the rat hepatic epoxide hydratase was measured in extrahepatic cholestasis induced by common bile duct (CBD) ligation after chronic ethanol intoxication. The liver microsomal epoxide hydratase activity in CBD ligated rats combined with chronic ethanol intoxication was lower than that in CBD ligation alone. On the other hands, the values of Vmax of the liver microsomal epoxide hydratase in CBD ligated rats combined with chronic ethanol intoxication was lower than that in CBD ligation alone. However, the value of Km of the above hepatic enzyme did not vary in the all experimental groups. This results indicate that the biosynthesis of the hepatic epoxide hydratase decreases in chronic ethanol intoxication combined with cholestasis than in cholestasis alone. Accordingly, the results will be the data supporting that alcoholic ingestion is enzymologically harmful in hepatobiliary disease.