간외성 담즙울체 쥐에서 간의 Epoxide Hydratase활성에 미치는 급성주정중독의 영향

Abstract

To elucidate the biochemical background of alcohol drinking hazard under the hepatobiliary disease, hepatic epoxide hydratase activity was determined in acute ethanol intoxicated rats with extrahepatic cholestasis induced by common bile duct ligation (CBD). Liver microsomal epoxide hydratase activity in CBD ligated rats combined with acute ethanol intoxication was lower than that in CBD ligation alone. On the other hand, the Vmax value of the enzyme in these rats was lower than that in CBD ligation alone, wheras the Km value of the above hepatic enzyme did not vary among all the experimental groups. This result indicates that biosynthesis of the hepatic epoxide hydratase decreases in acute ethanol intoxication combined with cholestasis than in cholestasis alone. Accordingly, the results support the observation that alcoholic drink is enzymologically harmful in hepatobiliary diseases.