골수 단핵세포의 거핵구 집락 배양에 대한 조혈성장인자의 효과

Abstract

In vitro colony assay procedures for expansion of human erythroid and granulocyte-macrophage progenitors have been used for many years. However,the development of reproducible assay method for human megakaryocyte (MK) progenitors considerably lagged behind. Cytokines such as stem cell factor (SCF), thrombopoietin (TPO), IL—6 and IL-11 have been demonstrated to have effects on the production of colony forming units (CFU)-MK. This study was undertaken to investigate the ex vivo expansion of megakaryocyte progenitors, derived from mononuclear cells of normal tone marrow. Bone marrow mononuclear cells were incubated in serum-free medium (MegaCult™, Stem Cell Technologies, Vancouver, Canada) and supplemented with SCF, granulocyte-colonystimulating factor (G-CSF), TPO, IL-11, combinations of SCF + G- CSF + TPO and SCF + G-CSF + TPO + IL-11. The effect of hematopoietic growth factors on ex vivo expansion of megakaryocyte progenitor cells was evaluated. These cytokines were able to generate of CFU-MK in the media and to increase megakaryocyte colony formation: Numbers of pure MK colonies increased in the media supplemented with SCF, IL-11 and combination of cytokines. Mixed MK colonies also increased in the media supplemented with SCF, G-CSF and combination groups.