총담관 대정맥문합수술을 한 쥐에서 혈청과 간의 라이소좀 α-D-Glucosidase 및 β-D-Glucuronidase 활성도에 미치는 Taurocholic Acid의 정맥 내 투여의 효과

Abstract

The effects of intravenous administration of high concentration of taurocholic acid (TCA) on α-D-glucosidase nad β-D-glucuronidase activities in rat liver lysosomes were studied. Above liver lysosomal enzymes, serum lysosmal α-D-Glucosidase and total β-D-Glucosidase activities were determined from the experimental rats with choledocho-caval shunt (CCS). The activities of liver lysosomal α-D-Glucosidase and β-D-Glucosidase as well as the activities of serum lysosomal α-D-Glucosidase and total β-D-Glucosidase were significantly increased in the CCS plus TCA injection group than in control group, such as group of CCS alone group. However, these hepatic and serum enzymes activities did not change in the CCS plus tauroursodeoxycholic acid injection group. The above results suggest that TCA stimulates the biosynthesis of the lysosomal α-D-Glucosidase and β-D-Glucosidase in the liver. And that the elevated lysosomal α-D-Glucosidase and total β-D-Glucosidase activities in the serum are most likely due to increased hepatocyte membrane permeability caused by TCA mediated liver cell necrosis.