3T3-L1 지방세포주에서 Epigallocatechin gallate가 세포증식 및 분화에 미치는 영향

Other Titles
Effect of Epigallocatechin Gallate on Cell Proliferation and Differentiation in 3T3-L1 Adipocytes
Authors
박재형배재훈박원균송대규
Department
Dept. of Medical Education (의학교육학); Dept. of Physiology (생리학
Citation
Keimyung Medical Journal, Vol.28(별호) : 100-110, 2009
Abstract
Green tea extracts contain many biologically-active materials, including catechins. The major water extractable component is epigallocatechin gallate (EGCG). (-)-Catechin at a higher concentration (50 μM) is also reported to be beneficial against obesity, by increasing adiponectin secretion from adipocytes. However, readily-achievable dose of EGCG in human blood after regular daily oral ingestion is around 1 μM, and (-)-catechin concentration is known to be further less than EGCG. Therefore, this study was designed to see whether they are real positive effecters against obesity at physiological blood levels, using a mouse adipocyte cell line, 3T3-L1. 3T3-L1 preadipocytes in Dulbecco's modified Eagles's medium (DMEM) media were differentiated by a serial 2-day addition method of isobutylmethylxanthine (IBMX), dexamethasone and insulin solution (Day 1 & 2) and insulin solution (Day 3 & 4). Each EGCG and (-)-catechins at concentrations between 0.1-10 μM was applied everyday from Day 1. Mitotic clonal expansion and expression level of C/EBPβwere measured at day 2, 2 days after the MDI treatment. Oil red-O staining was performed at Day 7, 8, 11. Adiponectin secretion measurement and Western blot for adiponectin and PPARγwere done at Day 8. EGCG and (-)-catechins were not influential in clonal expansion and C/EBPβprotein expression. Triglyceride accumulation in mature adipocytes tended to increase in EGCG-treated group, but not in (-)-catechins group. Adiponectin secretion was not modified in EGCG and (-)- catechin-treated groups. In addition, the two catechins did not significantly change the protein expression level of adiponectin and PPARγ. These may suggest that there is no direct evidence that EGCG has a mechanism in adipocytes, applicable to preventing from obesity by a daily regular consumption.
Keywords
AdipocytesAdiponectinEpigallocatechin gallateObesityPPARγ
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/15475
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2009
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Medical Education (의학교육학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
Keimyung Author(s)
박재형; 배재훈; 송대규; 박원균
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