HL-60 Leukemia 세포주에서 초음파에 의한 세포자멸사 유도
- 서성일; 서민호; 백원기
- Alternative Author(s)
- Suh, Seong Il; Suh, Min Ho; Baek, Won Ki
- Publication Year
- Apoptosis; Leukemia; Lysosome; Sonication; Ultrasonic therapy
- To investigate a specific mechanism of apoptosis induced by sonication, we applied 20 kHz ultrasound to leukemia cell line HL-60 with different intensities (0-60 W/cm2) and time durations (0-100 sec). In accordance with previous reports, ultrasound treatment in HL-60 cells induced immediate cell death and delayed cell death which are associated with cell lysis and apoptosis, respectively. Delayed cell death of HL-60 was also detected 5 hours after sonication in our experiment. Detection of caspase activation by Western blot and sub-G1 accumulation by flow cytometry confirmed that apoptosis plays a role in delayed cell death induced by sonication in HL-60 cells. In addition, we found that decrease in lysosomes of HL-60 cells after sonication suggesting lysosomal rupture is involved in the mechanism of cell death induced by sonication.
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