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난자 내로의 동결보존제 유입 궤적 최적화 모델링

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Affiliated Author(s)
허윤석
Alternative Author(s)
Heo, Yun Seok
Journal Title
Keimyung Medical Journal
Issued Date
2013
Keyword
CPA toxicityCryopreservationCryoprotectants (CPAs)Osmotic injury
Abstract
The inevitable use of the cryoprotectants (CPAs) during cryopreservation affects the low
viability of the cryopreserved oocytes and pregnancy rates either through CPA toxicity or
osmotic injury. The goal of this study is to develop an optimized CPA loading profile for
minimizing the exposure to CPA and the volume change of oocyte. A computational simulation has
been conducted to rationally design protocols that minimize the exposure to cryoprotectant and
the volume changes of oocyte. Using a coupled differential equation with hydraulic conductivity, solute permeability, and reflection coefficient of oocyte, oocyte volume changes are predicted as
function of time with the changes of the external CPA concentration. The computational
simulation suggests that controlled CPA loading profile produces less volume changes and less
total amounts of CPA exposure than uncontrolled CPA loading profile. Specifically, the volumetric
change (less than 10%) was obtained with the linear CPA loading profile while the volumetric
change in the conventional step-wise CPA profile was over 30%. The reduced volume change
can reduce the cellular damage from the osmotic injury during the pretreatment for the
cryopreservation. Thus, I believe that this computational modeling with controlled CPA profile will eventually help future advances in assisted reproductive technologies and fertility
preservation as well as in single cell analysis technology.
Alternative Title
Modeling for the Optimization of the Loading of Cryoprotectants to Oocyte
Department
Dept. of Biomedical Engineering (의용공학과)
Publisher
Keimyung University School of Medicine
Citation
Keimyung Medical Journal, Vol.32(2) : 161-167, 2013
Type
Article
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/15945
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2013
1. School of Medicine (의과대학) > Dept. of Biomedical Engineering (의용공학과)
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