Withaferin A enhances radiation-induced apoptosis in Caki cells through induction of reactive oxygen species, Bcl-2 downregulation and Akt inhibition

Title
Withaferin A enhances radiation-induced apoptosis in Caki cells through induction of reactive oxygen species, Bcl-2 downregulation and Akt inhibition
Authors
Eun Sun YangMin Jung ChoiJin Hee KimKyeong Sook ChoiTaeg Kyu Kwon
Keimyung Author(s)
권택규; 김진희
Department
Dept. of Immunology (면역학); Dept. of Radiation Oncology (방사선종양학)
Issue Date
2011
Publisher
School of Medicine
Citation
Chemico-Biological Interactions, Vol.190(1) : 9-15, 2011
Abstract
Withaferin A (Wit A), a natural compound derived from the medicinal plant Withania somnifera, has been reported for the anti-tumor effects, including the inhibition of tumor cell growth, metastasis and angiogenesis. In this study, we investigated the effect of Wit A on radiation-induced apoptosis in human renal cancer cells (Caki cells). Our results showed that, compared with Wit A or radiation alone, the combination of both resulted in a significant enhancement of apoptosis, showing the increase in the cleavage of caspase-3 and PARP as well as sub-G1 cell population. In addition, reactive oxygen species (ROS) generation was correlated with the enhancement of radiation-induced apoptosis by Wit A. Wit A downregulated Bcl-2 protein levels and ectopic expression of Bcl-2 in Caki cells attenuated the apoptosis induced by Wit A plus radiation. Taken together, these results indicate that Wit A enhanced radiation-induced apoptosis in Caki cells through ROS generation, down-regulation of Bcl-2 and Akt dephosphorylation. Thus, our study shows that Wit A may be used as an effective radiosensitizer in cancer therapy.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/17057
ISSN
0009-2797
Appears in Collections:
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학)
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