12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells

Title
12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells
Authors
BYEONG-CHURL JANGYU-KYOUNG PARKIN-HAK CHOISANG-PYO KIMJIN-BOK HWANGWON-KI BAEKMIN-HO SUHKYU-CHUL MUNSEONG-IL SUH
Keimyung Author(s)
장병철; 김상표; 황진복; 백원기; 서민호; 서성일; 문교철
Department
Dept. of Molecular Medicine (분자의학); Dept. of Pathology (병리학); Dept. of Pediatrics (소아청소년학); Dept. of Microbiology (미생물학); Dept. of Biochemistry (생화학); Chronic Disease Research Center (만성질환연구센터); Institute for Medical Science (의과학연구소)
Keywords
B7 family; 12-O-tetradecanoyl phorbol 13-acetate; Protein kinase C; Mitogen-activated protein kinases; Phosphatidylinositol- 3-kinase; K562 cells
Issue Date
2007
Publisher
School of Medicine
Citation
International Journal of Oncology, Vol.31(6) : 1439-1447, 2007
Abstract
Induction of the B7 family molecules by 12-Otetradecanoyl phorbol 13-acetate (TPA) has been reported, however, the mechanism by which TPA up-regulates these molecules remains poorly understood. In this study, the expression of B7-DC, -H1, -H2, and -H3 in response to TPA was markedly induced in K562 cells. TPA also induced activation of ERK, p38 mitogen-activated protein kinase (MAPK), JNK, phosphatidylinositol-3-kinase (PI-3K), or nuclear factor (NF)-κB. Pre-treatments with protein kinase C (PKC) inhibitors significantly inhibited TPA-induced expression of B7-DC, -H1, -H2, and -H3 mRNA as well as TPA-induced phosphorylation of ERK, p38 MAPK, JNK, and PI-3K. TPA-induced expression of B7-DC, -H1, -H2, and -H3 mRNA was abrogated by pre-treatments with inhibitors of ERK and p38 MAPK. However, inhibition of PI-3K and JNK only caused decrease of TPA-induced B7-DC mRNA and B7-H3 mRNA, respectively. TPA-induced degradation of IκB-α was markedly abrogated by treatments with PKC inhibitors, but not by treatments with inhibitors of ERK, p38 MAPK, JNK, or PI-3K. NF-κB inhibitors significantly attenuated the expression of B7-DC, -H1, -H2, and -H3 mRNA in response to TPA. These results suggest that TPA induces the expression of B7-DC, -H1, -H2, and -H3 mRNA in K562 cells via activation of PKC, ERK, p38 MAPK, and NF-κB. Distinctly, the expression of B7-DC mRNA and -H3 mRNA in response to TPA is also PI-3K- and JNK-dependent, respectively.
URI
https://www.spandidos-publications.com/10.3892/ijo.31.6.1439http://kumel.medlib.dsmc.or.kr/handle/2015.oak/17592
ISSN
1019-6439
Appears in Collections:
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Pediatrics (소아청소년학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. 연구논문 > 3. Researcher Institutues (부설연구소) > Chronic Disease Research Center (만성질환연구센터)
1. 연구논문 > 3. Researcher Institutues (부설연구소) > Institute for Medical Science (의과학연구소)
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