Aspirin induces apoptosis in YD-8 human oral squamous carcinoma cells through activation of caspases, down-regulation of Mcl-1, and inactivation of ERK-1/2 and AKT

Title
Aspirin induces apoptosis in YD-8 human oral squamous carcinoma cells through activation of caspases, down-regulation of Mcl-1, and inactivation of ERK-1/2 and AKT
Authors
In-Sook ParkJeong-Rang JoHua HongKi-Young NamJong-Bae KimSang-Hee HwangMi-Sun ChoiNam-Hee RyuHyun-Jung JangSang-Han LeeChin-Soo KimTae-Geon KwonGy-Young ParkJong-Wook ParkByeong-Churl Jang
Keimyung Author(s)
남기영; 김종배; 황상희; 박종욱; 장병철; 최미선; 류남희
Department
Dept. of Dentistry (치과); Dept. of Molecular Medicine (분자의학); Dept. of Immunology (면역학); Dept. of Pathology (병리학); Dept. of Laboratory Medicine (진단검사의학); Chronic Disease Research Center (만성질환연구센터)
Keywords
Aspirin; Apoptosis; YD-8 OSC cells; Caspases; Mcl-1; ERK-1/2; AKT; NF-κB
Issue Date
2010
Publisher
School of Medicine
Citation
TOXICOLOGY IN VITRO, Vol.24(3) : 713-720, 2010
Abstract
NSAIDs and COX-2 inhibitors show anti-cancer activities in many cancer cells. In this study, we investigated the effects of NSAIDs (aspirin or indomethacin) and COX-2 inhibitor (NS-398) on growth of YD-8 human oral squamous carcinoma cells. Interestingly, among drugs tested, aspirin showed strongest inhibitory effects on viability and survival of YD-8 cells. Profoundly, aspirin treatment resulted in severe cell shrinkage and nuclear DNA fragmentation in YD-8 cells, suggesting the aspirin-induced apoptosis in YD-8 cells. Data of Western blot further demonstrated that aspirin treatment caused activation of caspases, down-regulation of Mcl-1 protein, dephosphorylation of ERK-1/2 and AKT, and also IκB-α proteolysis-dependent NF-κB activation in YD-8 cells. Aspirin, however, had no effect on expressions of Bcl-2, XIAP, and HIAP-1 in YD-8 cells. Importantly, pretreatment with z-VAD-fmk, a pan-caspase inhibitor blocked the aspirin-induced apoptosis and Mcl-1 down-regulation in YD-8 cells. These findings collectively suggest that aspirin induces apoptosis in YD-8 cells and the induction may be correlated to activation of caspases, caspase-dependent Mcl-1 proteolysis, inactivation of ERK-1/2 and AKT, and activation of NF-κB. It is suggested that aspirin may be applied a potential anti-cancer drug against human oral squamous carcinoma.
URI
http://www.sciencedirect.com/science/article/pii/S0887233310000123/pdfft?md5=d31f1ea6bf3408d1f160feb0143ec44f&pid=1-s2.0-S0887233310000123-main.pdfhttp://kumel.medlib.dsmc.or.kr/handle/2015.oak/21161
ISSN
0887-2333
Appears in Collections:
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Dentistry (치과)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
1. 연구논문 > 3. Researcher Institutues (부설연구소) > Chronic Disease Research Center (만성질환연구센터)
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