cDNA Microarray를 이용한 HL60 세포주에서 방사선 조사 관련 유전자의 검색 및 분석

Title
cDNA Microarray를 이용한 HL60 세포주에서 방사선 조사 관련 유전자의 검색 및 분석
Other Titles
Analysis of X Irradiation Related Genes in HL60 Cells Using cDNA Microarray
Authors
박건욱황미선서성일서민호권택규박종욱조재위최은주백원기Keon Uk ParkMi-Sun HwangSeong-Il SuhMin-Ho SuhTaeg Kyu KwonJong-Wook ParkJae-We ChoEun-Ju ChoiWon-Ki Baek
Keimyung Author(s)
서성일; 서민호; 백원기; 조재위; 권택규; 박종욱
Department
Dept. of Microbiology (미생물학); Dept. of Dermatology (피부과학); Dept. of Immunology (면역학)
Keywords
Irradiation; HL60; cDNA microarray; Apoptosis
Issue Date
2000
Publisher
School of Medicine
Citation
대한미생물학회 The Korean Society For Microblology, Vol.35(4) : 299-308, 2000
Abstract
Recently developed cDNA microarray or DNA chip technology allows expression monitoring of expression of hundreds and thousands of genes simultaneously and provides a format for identifying genes as well as changes in their activity. In order to search for changes in gene expression after X irradiation in HL60 cells, cDNA microarray technique was done. In this study, expression of 588 human genes (including oncogenes, tumor suppressor genes, cell cycle regulator genes, intracellular signal transduction modulator genes, apoptosis related genes, transcription factor genes, growth factors and receptor genes, cytokine genes, etc)were analyzed. For cDNA microarray analysis mRNAs were extracted from control and 8 Gy-irradiated HL60 cells. As a result the changes in expression of several genes were observed. This alteration of gene expression was confirmed by reverse transcription-polymerase chain reaction. The expression of heat shock 60 KD protein, c-jun, erythroid protein kinase, interleukin-8, monocyte chemotactic peptide 1 and RANTES genes was increased, but the expression of p55CDC gene was decreased after X irradiation.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/23321
ISSN
0253-3162
Appears in Collections:
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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