HL60 세포주의 분화유도에 따른 Apoptosis관련 유전자의 발현

Title
HL60 세포주의 분화유도에 따른 Apoptosis관련 유전자의 발현
Other Titles
Expression of Apoptosis Related Genes During HL60 Cell Differentiation
Authors
백원기Won-Ki Baek
Keimyung Author(s)
백원기
Department
Dept. of Microbiology (미생물학)
Keywords
Differentiation; Apoptosis; HL60; bcl-2; bcl-x; c-iap 2; Procaspase 3
Issue Date
1999
Publisher
School of Medicine
Citation
대한미생물학회 The Korean Society For Microblology, Vol.34(1) : 85-92, 1999
Abstract
In hematopoietic tissues, apoptosis is coupled to terminal differentiation of myeloid progenitors. The mechanism of apoptosis associated with differentiation is poorly understood. HL60, promyelocytic leukemia cell line, was used to study expression of apoptosis related genes (bcl-2, bcl-xL/S, c-iap 2,and procaspase 3) during differentiation induced by 12-O-tetradecanoylphorbol- 13-acetate (TPA). HL60 cells were differentiated to monocyte/macrophage like cells about 1 day after TPA treatment, and differentiated cells were gradually induced apoptosis from 4 days after TPA treatment. The bcl-2 mRNA and protein product were rapidly and continuously downregulated but expression of bcl-xL and c-iap 2 genes were increased in early stage of differentiation and then decreased. Expression of bcl-xS, the alternative splicing form of bcl-xL gene, was slowly and continuously increased. These data suggest that increased expression of bcl-xL and c-iap 2 genes may provide a mechanism through which apoptosis is blocked in early stage of HL60 cell differentiation.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/23327
ISSN
0253-3162
Appears in Collections:
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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