PCR을 이용한 인간 세포거대바이러스(HCMV) DNA 중합효소 유전자의 검색

Title
PCR을 이용한 인간 세포거대바이러스(HCMV) DNA 중합효소 유전자의 검색
Other Titles
Detection of Human Cytomegalovirus DNA Polymerase Gene by Polymerase Chain Reaction
Authors
김현철박성배조원현백원기서민호Hyun Chul KimSung Bae ParkWon Hyun ChoWon Ki BaekMin Ho Suh
Keimyung Author(s)
김현철; 박성배; 조원현; 백원기; 서민호
Department
Dept. of Internal Medicine (내과학); Dept. of Surgery (외과학); Dept. of Microbiology (미생물학)
Keywords
Human Cytomegalovirus(HCMV); DNA polymerase gene; PCR; Southern blot
Issue Date
1992
Publisher
School of Medicine
Citation
대한미생물학회 The Korean Society For Microblology, Vol.27(2) : 181-188, 1992
Abstract
Polymerase chain reaction(PCF) amplification was used to detect human cytomegalovirus(HCMV) in MRC-5 cell culture. Oligonucleotide pairs for DNA polymerase gene of HCMV were used as primers and HCMV AD169 strain was used as a control. Amplified products were detected by gel electrophoresis and by Southern blot hybridization with digoxigenin-labeled HCMV DNA polymerase gene probe. In estimating the sensitivity of PCR, a 5㎕ aliquot of an infectivity titer of 10²³TCID₅₀/0.1㎖ of cell culture muxture was detected by direct agarose gel electrophoresis;South-ern blot hybridization was four-fold more sensitive(10¹·⁷TCID₅₀/0.1㎖) than gel analysis. The specificity of the PCR was evaluated using other members of the herpes family of viruses and various DANs. No amplification was noted by direct gel analysis or by dot blot hybridization and only HCMV containing specimen was positive. In conclusion, these results showed that the PCR may be a sensitive and fast tool for the diagno-sis of HCMV infections.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/23330
ISSN
0253-3162
Appears in Collections:
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
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