임상 검체에서 PCR을 이용한 수두-대상포진바이러스 DNA의 검색

Title
임상 검체에서 PCR을 이용한 수두-대상포진바이러스 DNA의 검색
Other Titles
Detection of Varicella-Zoster Virus DNA in Clinical Specimens by Polymerase Chain Reaction(PCR)
Authors
서민호백원기이규석Min Ho SuhWon Ki BaekKyu Suk Lee
Keimyung Author(s)
서민호; 백원기; 이규석
Department
Dept. of Microbiology (미생물학); Dept. of Dermatology (피부과학)
Keywords
Varicella-zoster virus; PCR; Southern blot; Slot blot
Issue Date
1991
Publisher
School of Medicine
Citation
대한미생물학회 The Korean Society For Microblology, Vol.26(5) : 479-486, 1991
Abstract
The polymerase chain reaction (PCR) was used to detect varicella-zoster virus (VZV) DNA in the herpes zoster vesicle specimens and paraffin section specimens from patients. Oligonucleotide pairs for 44 kilodalton protein gene were used as primers and VZV Oka strain was used as a control. Positive results were obtained in 10 of 10 vesicle specimens and 6 10 paraffin section specimens. Detection of PCR products by direct agarose gel electrophoresis (EP) showed positive results in 10 of 10 vesicle specimens and only 3 of 10 paraffin sections. But with southern blot hybri¬dization, we could obtain positive results in 10 of 10 vesicles and 6 of 10 paraffin sections. Slot blot hybridization data also showed the same results as in the case of southern blot analysis. Dig-oxigenin labeling and detection method was used in southern and slot blot hybridiations in order to obtain data rapidly and more conveniently. In conclusion, these results showed that the PCR provides a useful tool for the early diagnosis of VZV infections and the detection rate of PCR products is increased by agarose gel EP with southern or slot blot hybridization.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/23345
ISSN
0253-3162
Appears in Collections:
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
Files in This Item:
비공개 원문입니다.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE