뇌동맥류에서 혈관형성 인자와 혈관벽 기질 단백에 대한 면역조직화학적 연구

Other Titles
Immunohistochemical Study for the Angiogenesis Factors and Vascular Wall Matrix Proteins in Intracranial Aneurysms
Authors
김재홍임만빈이창영김상표
Keimyung Author(s)
임만빈; 이창영; 김상표
Department
Dept. of Neurosurgery (신경외과학); Dept. of Pathology (병리학)
Keywords
Aneurysm; Basic fibroblast growth factor; Vascular endothelial growth factor; Alpha smooth muscle actin; Collagen type IV
Issue Date
2000
Publisher
School of Medicine
Citation
대한신경외과학회지, Vol.29(12) : 1584-1591, 2000
Abstract
Objective:Until now, it has been little known about the biological mechanisms associated with the genesis, growth, and rupture of intracranial aneurysm. This study was performed to investigate and understand a part of these mechanisms. Materials and Methods:Immunohistochemical stains for angiogenesis growth factors(basic fibroblast growth factor(bFGF) and vascular endothelial growth factor(VEGF)) and selected vascular wall matrix proteins(alpha smooth muscle actin(αSMA) and collagen Type IV) were performed in fixed sections from a normal circle of Willis artery which was taken from the autopsy specimen as a control vessel and 17 aneurysmal wall specimens which was taken during surgical clipping of aneurysms. The staining intensity and distribution of immunoreactivity to angiogenesis growth factors and selected wall matrix proteins in control vessel and aneurysmal wall were examined and compared with each other. The difference of staining intensity according to the size of aneurysm was also investigated. Results:There was no immunoreactivity to bFGF and VEGF in the control vessel. bFGF immunoreactivity was exhibited in 15 of 17 aneurysm specimens around smooth muscle cells within the media of aneurysm. VEGF immunoreactivity was also exhibited in all aneurysm specimens in patches or diffusely affecting all layers of the aneurysmal wall. The degrees of intensity of bFGF and VEGF immunoexpression were proportionate roughly to the size of aneurysm. Strong immunoexpression of both factors were noticed in large aneurysm. A regularly arranged and defined band of immunoreactivity of αSMA was noticed in the media of the control vessel, whereas diffuse, faint, irregularly arranged αSMA was noticed in the aneurysmal wall. A regularly defined band of collagen Type IV immunoreactivity was also noticed in the subendothelium of the control vessel, whereas diffuse disorganized immunoreactivity of collagen Type IV was noticed in the entire wall of the aneurysm. Conclusion:These results indicate substantial evidences of abnormal expression of angiogenesis factors and changes of selected vascular wall matrix proteins in the wall of intracranial aneurysm. The unbalanced changes of angiogenesis factors and vascular wall matrix proteins in the wall of aneurysm may be one of the biological mechanisms for the growth and rupture of aneurysm.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/27424
ISSN
1225-8245
Appears in Collections:
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학)
1. 연구논문 > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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