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Repeated Water Avoidance Stress Alters Mucosal Mast Cell Counts, Interleukin-1β Levels with Sex Differences in the Distal Colon of Wistar Rats.

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Author(s)
이주엽
Alternative Author(s)
Lee, Ju Yup
Issued Date
2016
Keyword
CytokinesMast cellsWater avoidance stress
Abstract
Background/Aims:

This study was aimed at evaluating differences in the effects of repeated water avoidance stress (rWAS) on colonic movement, mucosal mast cell counts, cytokine levels, and visceromotor response (VMR) to colorectal distension (CRD) in rats of both sexes.


Methods:

Wistar rats were divided into stress and no-stress groups. Rats in the stress group were exposed to rWAS (1 hr/day) for 10 days. Mucosal mast cells were immunohistochemically stained with anti-mast cell tryptase antibody and counted. The colonic mucosal cytokine levels were measured with enzyme-linked immunosorbent assay. The VMR to CRD (visceral analgesia) was assessed by using a barostat and noninvasive manometry.


Results:

The mean number of fecal pellets in the rWAS group increased significantly as compared with that in the no-stress group in both sexes. After adjustment for body weight, the female rats had a significantly higher pellet output than the male rats. The mucosal mast cell count of the female rWAS group was higher than that of the male rWAS group (13.0 ± 0.9 vs 8.8 ± 0.6; P < 0.001). The colonic mucosal interleukin-1β level was also higher only in the female rats of the rWAS group than in those of the no-stress group. On days 10 and 11, a decrease in VMR to CRD was observed at 40 and 60 mmHg in both sexes of the rWAS group, without a sex-based difference.


Conclusions:

The colonic response to stress appeared to be more sensitive in the female rats than in the male rats. However, stress-induced visceral analgesia had no sex-related difference and the underlying mechanism needs to be further evaluated.
Department
Dept. of Internal Medicine (내과학)
Publisher
School of Medicine
Citation
Journal of Neurogastroenterology and motility, Vol.22(4) : 694-704, 2016
Type
Article
ISSN
2093-0879
DOI
10.5056/jnm16007
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/32434
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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