Jaceosidin induces apoptosis through Bax activation and down-regulation of Mcl-1 and c-FLIP expression in human renal carcinoma Caki cells

Authors
Seon Min WooTaeg Kyu Kwon
Department
Dept. of Immunology (면역학)
Issue Date
2016
Citation
Chemico-Biological Interactions, Vol.260(25) : 168-175, 2016
ISSN
0009-2797
Abstract
Jaceosidin is a flavonoid isolated from Artemisia vestita that has been reported to possess anti-tumor and anti-proliferative activities in many cancer cells. In this study, we investigated the anti-tumor activity of jaceosodin in renal carcinoma cells. Jaceosidin induced apoptosis in multiple human renal carcinoma cells (Caki, ACHN, A498, and 786-O), lung cancer cells (A549) and glioma cells (U251MG). In contrast, jaceosidin does not induce apoptosis in normal human umbilical vein cells (EA.hy926). Apoptotic cell death was associated with the activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase. Treatment with jaceosidin also caused loss of mitochondrial membrane potential (MMP) and Bax activation, which led to the release of cytochrome c into the cytosol. We also found that jaceosidin downregulated Mcl-1 and c-FLIP expression at the transcriptional level and that ectopic expression of Mcl-1 and c-FLIP blocked jaceosidin-induced apoptosis. Cumulatively, our results suggest that jaceosidin induces apoptosis in renal carcinoma cells through Bax activation and reduces Mcl-1 and c-FLIP expression.
Keywords
JaceosidinApoptosisBaxc-FLIPMcl-1Renal cancer
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/32735
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
Keimyung Author(s)
권택규
Full Text
https://linkinghub.elsevier.com/retrieve/pii/S0009279716304434
File in this Item
 사서에게 요청하기
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE