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comparison of the automated cobas u 701 urine microscopy and UF-1000i flow cytometry systems and manual microscopy in the examination of urine sediments

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Affiliated Author(s)
하정숙류남희이원목
Alternative Author(s)
Ha, Jung SookRyoo, Nam HeeLee, Won Mok
Journal Title
Journal of Clinical Laboratory Analysis
ISSN
0887-8013
Issued Date
2016
Keyword
automated urine sediment analyzercobas u 701UF-1000iurine microscopyurine sediment
Abstract
Background: The cobas u 701, a new au-
tomated image-based urine sediment ana-
lyzer, was introduced recently. In this study,
we compared its performance with that of
UF-1000i flow cytometry and manual mi-
croscopy in the examination of urine sed-
iments.
Methods: Precision, linearity, and
carry-over were determined for the two urine
sediment analyzers. For a comparison of
the method, 300 urine samples were ex-
amined by the automated analyzers and by
manual microscopy using a KOVA chamber.
Results: Within-run coefficients of variation
(CVs) for the control materials were 7.0–
8.8% and 1.7–5.7% for the cobas u 701 and
UF-1000i systems, respectively. Between-
run CVs were 8.5–9.8% and 2.7–5.4%, re-spectively. Both instruments showed good
linearity and negligible carry-over. For red
blood cells (RBC), white blood cells (WBC),
and epithelial cells (EPI), the overall concor-
dance rates within one grade of difference
among the three methods were good (78.6–
86.0%, 88.7–93.8%, and 81.3–90.7%, re-
spectively). The concordance rate for casts
was poor (66.5–68.9%).
Conclusion: Com-
pared with manual microscopy, the two au-
tomated sediment analyzers tested in this
study showed satisfactory analytical perfor-
mances for RBC, WBC, and EPI. However,
for other urine sediment particles confirma-
tion by visual microscopy is still required.
Department
Dept. of Laboratory Medicine (진단검사의학)
Publisher
School of Medicine
Citation
Wonmok Lee, Jung‐Sook Ha, Nam‐Hee Ryoo. (2016). comparison of the automated cobas u 701 urine microscopy and UF-1000i flow cytometry systems and manual microscopy in the examination of urine sediments. Journal of Clinical Laboratory Analysis, 30(5), 663–671. doi: 10.1002/jcla.21919
Type
Article
ISSN
0887-8013
DOI
10.1002/jcla.21919
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/32784
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
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