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High expression of microRNA-196a indicates poor prognosis in resected pancreatic neuroendocrine tumor

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Affiliated Author(s)
이윤석
Alternative Author(s)
Lee, Yoon Suk
Journal Title
Medicine
ISSN
0025-7974
Issued Date
2015
Abstract
There is limited data on miRNA expression in pancreatic
neuroendocrine tumors (PanNETs). In this study, we aimed to identify
miRNAs that could be potential prognostic biomarkers of PanNETs in
patients who underwent curative surgery.
For miRNA target screening, 2 primary PanNETs and corresponding
liver metastases were screened for miRNA expression by the
NanoString nCounter analysis. Candidate miRNAs were selected by
2-fold difference of expression between metastatic versus primary
tumor. For miRNA target validation, quantitative real-time PCR was
performed for candidate miRNAs on 37 PanNETs and matched nonneoplastic
pancreata, and the miRNA levels were correlated with the
clinicopathological features and patient survival data.
Eight miRNAs (miRNA-27b, -122, -142–5p, -196a, -223, -590–5p, -
630, and -944) were selected as candidate miRNAs. OnlymiR-196a level
was significantly associated with stage, and mitotic count. When Pan-
NETs were stratified into high (n¼10) and low (n¼27) miRNA-196a
expression groups, miRNA-196a-high PanNETs were significantly
associated with advanced pathologic T stage (50.0% vs 7.4%), N stage
(50.0% vs 3.7%), higher mitotic counts (60.0% vs 3.7%), and higher Ki-
67-labeling indices (60.0% vs 22.2%). In addition, high miRNA-196a
expression was significantly associated with decreased overall survival
(P¼0.046) and disease-free survival (P<0.001) during a median followup
of 37.9 months with the hazard ratio for recurrence of 16.267 (95%
confidence interval¼1.732–153.789; P¼0.015).
MiRNA-196a level may be a promising prognostic marker of recurrence
in resected PanNETs, although further experimental investigation
would be required.
Department
Dept. of Internal Medicine (내과학)
Publisher
School of Medicine
Citation
Yoon Suk Lee et al. (2015). High expression of microRNA-196a indicates poor prognosis in resected pancreatic neuroendocrine tumor. Medicine, 94(50), e2224–e2224. doi: 10.1097/MD.0000000000002224
Type
Article
ISSN
0025-7974
DOI
10.1097/MD.0000000000002224
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/32973
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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