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Roux-en-Y gastric bypass stimulates hypothalamic miR-122 and inhibits cardiac and hepatic miR-122 expressions

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Affiliated Author(s)
권인규류승완하은영
Alternative Author(s)
Kwon, In GyuRyu, Seung WanHa, Eun Young
Journal Title
Journal of Surgical Research
ISSN
0022-4804
Issued Date
2015
Keyword
Roux-en-Y gastric bypassMicroRNA-122AMP-activated protein kinase
Abstract
Background: MicroRNAs (miRNAs) are endogenous noncoding small ribonucleic acids that
have emerged as one of the central players of gene expression regulation. This study was
designed to determine and identify miRNAs that are associated with Roux-en-Y gastric
bypass (RYGB).
Methods: Male SpragueeDawley rats were divided into two groups: sham and RYGB.
Changes in food intake and body weight were measured. miRNA microarray analyses on
the brain hypothalamus and heart were performed. The expressions of miR-122 were
analyzed, and the activities of adenosine monophosphateeactivated protein kinase (AMPK)
were determined in the hypothalamus, heart, and liver. Antisense oligonucleotide miR-122
was transfected into hepatocellular carcinoma cells to validate in vivo results.
Results: Body weights decreased in the RYGB group compared with those in sham group.
Food intake was different between sham and RYGB groups. Of 350 miRNAs that were
investigated, we observed that miR-122, being predominantly found in the liver, markedly
increased (>35-fold) in the hypothalamus and decreased (>4-fold) in the heart. Quantitative
polymerase chain reaction analysis revealed that expression of miR-122 was induced
in hypothalamus but attenuated in the heart and liver of the RYGB group. Activities of
AMPK were decreased in the hypothalamus but increased in the heart and liver. Knockdown
of miR-122 in hepatocellular carcinoma cells stimulated phosphorylation levels
AMPK.
Conclusions: The results in this study suggest that RYGB regulates the expressions of miR-
122 in the hypothalamus, heart, and liver, which in turn may modulate the activities of
AMPK, the master regulator of metabolism.
Department
Dept. of Surgery (외과학)
Dept. of Biochemistry (생화학)
Institute for Cancer Research (암연구소)
Publisher
School of Medicine
Citation
In Gyu Kwon et al. (2015). Roux-en-Y gastric bypass stimulates hypothalamic miR-122 and inhibits cardiac and hepatic miR-122 expressions. Journal of Surgical Research, 199(2), 371–377. doi: 10.1016/j.jss.2015.05.055
Type
Article
ISSN
0022-4804
DOI
10.1016/j.jss.2015.05.055
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33044
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
3. Research Institutues (연구소) > Institute for Cancer Research (암연구소)
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