An enzymatically fortified ginseng extract inhibits proliferation and induces apoptosis of KATO3 human gastric cancer cells via modulation of Bax, mTOR, PKB and IκBα.

Authors
JEONG‑WON HWANGYOUNG‑MI BAEKIK‑SOON JANGKYEONG EUN YANGDONG‑GI LEESO‑JUNG YOONJAERANG RHOCHONG‑KWAN CHOYEON‑WEOL LEEKI‑ROK KWONHWA‑SEUNG YOOJUNG‑SUK SUNGSHIN KIMJONG‑WOOK PARKBYEONG‑CHURL JANGJONG‑SOON CHOI
Department
Dept. of Immunology (면역학); Dept. of Molecular Medicine (분자의학)
Issue Date
2015
Citation
Molecular Medicine Reports, Vol.11(1) : 670-676, 2015
ISSN
1791-2997
Abstract
Accumulative evidence suggests ginseng extract and/or its major components, ginsenosides and compound K, a metabolized ginseng saponin, have anti-cancer effects. In the present study, the effects of a ginseng butanolic extract (GBX) and an enzymatically fortified ginseng extract (FGX), with enriched ginsenosides and compound K, on the growth of KATO3 human gastric cancer cells were investigated using a cell viability assay. While treatment with GBX at 31.25-125 mg/ml for 24 h did not affect the proliferation of KATO3 cells, FGX under the same conditions inhibited cell proliferation in a concentration-dependent manner. Furthermore, Annexin V/PI-staining and flow cytometric analysis demonstrated that the population of apoptotic KATO3 cells was increased following treatment with FGX, which was greater than in the GBX-treated cells, suggesting that FGX had a stronger apoptotic effect than GBX. To investigate the underlying mechanism of the cytostatic and cytotoxic effects of the ginseng extracts, apoptosis-associated proteins were assessed using western blot analysis. The data revealed higher expression levels of B-cell lymphoma 2-associated X protein (Bax), lower expression of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α (IκBα) and reduced phosphorylation of mammalian target of rapamycin (mTOR) and protein kinase B (PKB) in the FGX-treated KATO3 cells than in the GBX-treated cells. Collectively, these results demonstrated for the first time, to the best of our knowledge, that FGX had stronger anti-proliferative and pro-apoptotic effects on KATO3 cells than GBX. The anti-proliferative and/or pro-apoptotic effects of FGX appeared to be mediated via the upregulation of Bax, IκBα proteolysis (activation of nuclear factor-κB) and the blocking of mTOR and PKB signals. Key words: fortified ginseng extract, Bax, IκBα, mTOR, PKB, KATO3 cells
Keywords
fortified ginseng extractBaxIκBαmTORPKBKATO3 cells
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/33446
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
Keimyung Author(s)
김신; 박종욱; 장병철
Full Text
http://lps3.www.spandidos-publications.com.proxy.dsmc.or.kr/mmr/11/1/670
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