Application of platelet-rich plasma accelerates the wound healing process in acute and chronic ulcers through rapid migration and upregulation of cyclin A and CDK4 in HaCaT cells

Authors
SUNG-AE KIMHAN-WON RYUKYU-SUK LEEJAE-WE CHO
Department
Dept. of Dermatology (피부과학)
Issue Date
2013
Citation
Molecular Medicine Reports, Vol.7(2) : 476-480, 2013
ISSN
1791-2997
Abstract
Application of autologous platelet-rich plasma (PRP) has been used for chronic wound healing. The aim of this study was to evaluate the effect of PRP on the wound healing processes of both acute and chronic ulcers and the underlying molecular mechanisms involved. We treated 16 patients affected by various acute and chronic ulcers with PRP. We performed molecular studies of cell proliferation, migration assays, immunoblotting and chloramphenicol acetyltransferase (CAT) assays in PRP-treated HaCaT keratinocyte cells. PRP treatment induced increased rates of cell proliferation and cell migration of HaCaT cells. In addition, the expression of cyclin A and cyclin dependent kinase (CDK) 4 proteins was markedly increased with a low concentration (0.5%) of PRP treatment in HaCaT cells. In 11 patients with chronic ulcers, including stasis ulcers, diabetic ulcers, venous leg ulcers, livedoid vasculitis, claw foot and traumatic ulcers, 9 patients showed 90-100% epithelization after 15.18 days. In 5 patients with acute ulcers, such as dehiscence, open wound and burn wound, 80-100% epithelization was achieved between 4 to 20 days. Topical application of PRP to acute and chronic skin ulcers significantly accelerated the epithelization process, likely through upregulation of the cell cycle regulatory proteins cyclin A and CDK4. Key words: platelet-rich plasma, skin ulcer, keratinocytes, cell cycles
Keywords
platelet-rich plasmaskin ulcerkeratinocytescell cycles
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/33459
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
Keimyung Author(s)
김성애; 이규석; 조재위
Full Text
http://lps3.www.spandidos-publications.com.proxy.dsmc.or.kr/mmr/7/2/476
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