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Gene knockdown by large circular antisense for high-throughput functional genomics

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Affiliated Author(s)
강구정박종욱박종구
Alternative Author(s)
Kang, Koo JeongPark, Jong WookPark, Jong Gu
Journal Title
Nat Biotechnol
ISSN
1087-0156
Issued Date
2005
Abstract
Single-stranded genomic DNA of recombinant M13 phages was tested as an antisense molecule and examined for its
usefulness in high-throughput functional genomics. cDNA fragments of various genes (TNF-a, c-myc, c-myb, cdk2 and cdk4)
were independently cloned into phagemid vectors. Using the life cycle of M13 bacteriophages, large circular (LC)-molecules,
antisense to their respective genes, were prepared from the culture supernatant of bacterial transformants. LC-antisense
molecules exhibited enhanced stability, target specificity and no need for target-site searches. High-throughput functional
genomics was then attempted with an LC-antisense library, which was generated by using a phagemid vector that incorporated
a unidirectional subtracted cDNA library derived from liver cancer tissue. We identified 56 genes involved in the growth of
these cells. These results indicate that an antisense sequence as a part of single-stranded LC-genomic DNA of recombinant
M13 phages exhibits effective antisense activity, and may have potential for high-throughput functional genomics.
Department
Dept. of Surgery (외과학)
Dept. of Immunology (면역학)
Dept. of Molecular Medicine (분자의학)
Publisher
School of Medicine
Citation
Yun-Han Lee et al. (2005). Gene knockdown by large circular antisense for high-throughput functional genomics. Nat Biotechnol, 23(5), 591–599.
Type
Article
ISSN
1087-0156
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33468
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
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