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Afterhyperpolarization induced by the activation of nicotinic acetylcholine receptors in pelvic ganglion neurons of male rats

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Author(s)
Kyu-Sang ParkSeung-Kuy ChaMin-Jeong KimNa-Hyun KimJoong-Woo LeeSeong-Woo JeongIn Deok Kong
Publication Year
2010
Keyword
Nicotinic acetylcholine receptorAutonomic gangliaAfterhyperpolarizationSodium/potassium ATPaseCa2+-activated K+ channel
Abstract
The electrophysiological mechanism underlying afterhyperpolarization induced by the activation of the nicotinic acetylcholine receptor (nAChR) in male rat major pelvic ganglion neurons (MPG) was investigated using a gramicidin-perforated patch clamp and microscopic fluorescence measurement system. Acetylcholine (ACh) induced fast depolarization through the activation of nAChR, followed by a sustained hyperpolarization after the removal of ACh in a dose-dependent manner (10 μM to 1 mM). ACh increased both intracellular Ca2+ ([Ca2+]i) and Na+ concentrations ([Na+]i) in MPG neurons. The recovery of [Na+]i after the removal of ACh was markedly delayed by ouabain (100 μM), an inhibitor of Na+/K+ ATPase. Pretreatment with ouabain blocked ACh-induced hyperpolarization by 67.2 ± 5.4% (n = 7). ACh-induced hyperpolarization was partially attenuated by either the chelation of [Ca2+]i with BAPTA/AM (20 μM) or the blockade of small-conductance Ca2+-activated K+ channels by apamin (500 nM). Taken together, the activation of nAChR increases [Na+]i and [Ca2+]i, which activates Na+/K+ ATPase and Ca2+-activated K+ channels, respectively. Consequently, hyperpolarization occurs after the activation of nAChR in the autonomic pelvic ganglia.

Abbreviations
ACh, acetylcholine;
MPG, major pelvic ganglion;
nAChR, nicotinic acetylcholine receptor;
[Ca2+]i, intracellular calcium ion concentration;
[Na+]i, intracellular sodium ion concentration

Keywords
Nicotinic acetylcholine receptor;
Autonomic ganglia;
Afterhyperpolarization;
Sodium/potassium ATPase;
Ca2+-activated K+ channel
Department
Dept. of Nursing (간호학)
Publisher
College of Nursing
Citation
Neuroscience Letters, Vol.482(2) : 167-171, 2010
Type
Article
ISSN
0304-3940
DOI
10.1016/j.neulet.2010.07.026
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/33542
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