AP-1 transcription factor decoy reduces the TGF-β1-induced cell growth in scleroderma fibroblasts through inhibition of cyclin E

Authors
JAE-WE CHOYOUNG-CHANG CHAKYU-SUK LEE
Department
Dept. of Dermatology (피부과학)
Issue Date
2008
Citation
Oncology Reports, Vol.19(3) : 737-741, 2008
ISSN
1021-335X
Abstract
The transforming growth factor-ß (TGF-ß) signaling pathway plays a key role in the abnormal accumulation of type I and III collagen of scleroderma. Activator protein-1 (AP-1) is a key regulatory protein in TGF-ß1-induced type I collagen synthesis. However, it is largely unknown whether AP-1 is involved in the cell proliferation of fibroblasts in scleroderma. In this study, we investigated the effects of the AP-1 oligo-deoxynucleotide (ODN) decoy on TGF-ß1- induced cell growth in scleroderma fibroblasts. To investigate the inhibition of AP-1 ODN decoy on the growth rates of scleroderma fibroblasts through the regulation of cell cycle regulatory proteins, we transfected the AP-1 ODN decoy on scleroderma fibroblasts and analyzed the cell cycle regulatory proteins by RT-PCR and Western blot analysis. We found that the growth rates of normal fibroblasts and scleroderma fibroblasts showed similar rates. It is noteworthy that the scleroderma fibroblasts grew more rapidly than normal fibroblasts in the presence of TGF-ß1. Moreover, the transfection of AP-1 decoy ODN into scleroderma fibroblasts resulted in the down-regulation of the growth rates by the down-regulation of cyclin E. These results collectively suggest that AP-1 ODN decoy can down-regulate the growth rates of scleroderma fibroblasts, thus implying that AP-1 ODN decoy is a promising therapeutic tool for overcoming scleroderma. Key words: scleroderma, transforming growth factor-ß, activator protein-1 oligodeoxynucleotides decoy, cyclin E
Keywords
sclerodermatransforming growth factor-ßactivator protein-1 oligodeoxynucleotides decoycyclin E
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/33607
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
Keimyung Author(s)
조재위; 이규석
Full Text
https://www.spandidos-publications.com/or/19/3/737
File in this Item
 사서에게 요청하기
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE