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AP-1 transcription factor decoy reduces the TGF-β1-induced cell growth in scleroderma fibroblasts through inhibition of cyclin E

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Affiliated Author(s)
조재위이규석
Alternative Author(s)
Cho, Jae WeLee, Kyu Suk
Journal Title
Oncology Reports
ISSN
1021-335X
Issued Date
2008
Keyword
sclerodermatransforming growth factor-ßactivator protein-1 oligodeoxynucleotides decoycyclin E
Abstract
The transforming growth factor-ß (TGF-ß) signaling
pathway plays a key role in the abnormal accumulation of
type I and III collagen of scleroderma. Activator protein-1
(AP-1) is a key regulatory protein in TGF-ß1-induced type I
collagen synthesis. However, it is largely unknown whether
AP-1 is involved in the cell proliferation of fibroblasts in
scleroderma. In this study, we investigated the effects of the
AP-1 oligo-deoxynucleotide (ODN) decoy on TGF-ß1-
induced cell growth in scleroderma fibroblasts. To investigate
the inhibition of AP-1 ODN decoy on the growth rates of
scleroderma fibroblasts through the regulation of cell cycle
regulatory proteins, we transfected the AP-1 ODN decoy on
scleroderma fibroblasts and analyzed the cell cycle
regulatory proteins by RT-PCR and Western blot analysis. We
found that the growth rates of normal fibroblasts and
scleroderma fibroblasts showed similar rates. It is noteworthy
that the scleroderma fibroblasts grew more rapidly than normal
fibroblasts in the presence of TGF-ß1. Moreover, the
transfection of AP-1 decoy ODN into scleroderma fibroblasts
resulted in the down-regulation of the growth rates by the
down-regulation of cyclin E. These results collectively
suggest that AP-1 ODN decoy can down-regulate the growth
rates of scleroderma fibroblasts, thus implying that AP-1 ODN
decoy is a promising therapeutic tool for overcoming
scleroderma.
Key words: scleroderma, transforming growth factor-ß, activator
protein-1 oligodeoxynucleotides decoy, cyclin E
Department
Dept. of Dermatology (피부과학)
Publisher
School of Medicine
Citation
JAE-WE CHO et al. (2008). AP-1 transcription factor decoy reduces the TGF-β1-induced cell growth in scleroderma fibroblasts through inhibition of cyclin E. Oncology Reports, 19(3), 737–741. doi: 10.3892/or.19.3.737
Type
Article
ISSN
1021-335X
DOI
10.3892/or.19.3.737
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33607
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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