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Modification of gene expression by melatonin in UVB-irradiated HaCaT keratinocyte cell lines using a cDNA microarray

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Affiliated Author(s)
조재위김창욱이규석
Alternative Author(s)
Cho, Jae WeKim, Chang WookLee, Kyu Suk
Journal Title
Oncology Reports
ISSN
1021-335X
Issued Date
2007
Keyword
melatoninUVBkeratinocytescDNA microarray
Abstract
Excessive ultraviolet B (UVB) irradiation causes
apoptotic cell death or induction of tumors in skin. Melatonin is
a promising antioxidant and direct radical scavenger. Recently,
it was reported that melatonin increases the survival of ultraviolet-
B (UVB)-irradiated HaCaT keratinocyte cell lines.
However, the precise molecular mechanisms underlying
protective effect of melatonin on UVB damage are largely
unknown. In this study, to gain more insight into the molecular
mechanisms involved in melatonin-induced cell survival on
UVB-irradiated HaCaT keratinocytes, we performed cDNA
microarray analysis. HaCaT keratinocytes were incubated
without or with melatonin at 100 nm for 30 min prior to UVB
irradiation at 100 mJ/cm2, and total RNA was isolated. Our
data showed that the expression of apoptosis regulator genes
(apoptosis related protein-3, apoptotic chromatin condensation
inducer in the nucleus), cancer related genes (tumor suppressor
deleted in oral cancer-related 1), cell cycle regulator (cyclindependent
kinase 2 interacting protein), enzymes (glutathione
peroxidase 1, ubiquitin-conjugating enzyme E2M), and signal
transducer genes [fibroblast growth factor (acidic) intracellular
binding protein, transforming growth factor ß-stimulated
protein TSC-22] were decreased by melatonin treatment in
the UVB-irradiated HaCaT keratinocyte cell lines, compared
to that of UVB-irradiated HaCaT cells without melatonin.
Thus, findings of the present study demonstrate that melatonin
modulates the expression of apoptosis related genes in UVBirradiated
HaCaT cells, resulting in increasing cell survival,
thereby suggesting that melatonin may be used as a promising
sunscreen substance to reduce cell death of keratinocytes
after excessive UVB irradiation.
Key words: melatonin, UVB, keratinocytes, cDNA microarray
Department
Dept. of Dermatology (피부과학)
Publisher
School of Medicine
Citation
JAE-WE CHO et al. (2007). Modification of gene expression by melatonin in UVB-irradiated HaCaT keratinocyte cell lines using a cDNA microarray. Oncology Reports, 17(3), 573–577. doi: 10.3892/or.17.3.573
Type
Article
ISSN
1021-335X
DOI
10.3892/or.17.3.573
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33611
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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