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Protective effect of green tea polyphenol (-)-epigallocatechin gallate and other antioxidants on lipid peroxidation in gerbil brain homogenates

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Author(s)
이성용서성일정정길
Alternative Author(s)
Lee, Seong RyongSuh, Seong IlChung, Jung Kil
Publication Year
2003
Keyword
EGCGtroloxlipoic acidmelatoninbrain homogenateantioxidantlipid peroxidation
Abstract
The aim of this study was to compare the protective effects of green tea polyphenol (-)-epigallocatechin gallate
(EGCG) and other well-known antioxidants on the lipid peroxidation in gerbil brain homogenates. Oxidative
stress was induced by H2O2 (10 mM) or ferrous ammonium sulfate (5 μM) and lipid peroxidation was
studied. Hydrogen peroxide and ferrous ions are capable of oxidizing a wide range of substrates and causing
biological damage. The reaction, referred to as the Fenton process, is complex and can generate both
hydroxyl radicals and higher oxidation states of the iron. Thiobarbituric acid-reactive substances (TBA-RS)
were used as a marker of lipid peroxidation. EGCG, trolox, lipoic acid, and melatonin reduced H2O2- or ferrous
ion-induced lipid peroxidation in a concentration-dependant manner. In reducing the H2O2-induced
lipid peroxidation, IC50 values of antioxidants were as follows: EGCG (0.66 μM), trolox (37.08 μM), lipoic
acid (7.88 mM), and melatonin (19.11 mM). In reducing the ferrous ion-induced lipid peroxidation, IC50
values of antioxidants were as follows: EGCG (3.32 μM), trolox (75.65 μM), lipoic acid (7.63 mM), and melatonin
(15.48 mM). Under the in vitro conditions of this experiment, EGCG was the most potent antioxidant in
inhibiting H2O2 or ferrous ion-induced lipid peroxidation in the gerbil brain homogenates. Copyright © 2003
John Wiley & Sons, Ltd.
Keywords: EGCG; trolox; lipoic acid; melatonin; brain homogenate; antioxidant; lipid peroxidation.
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