Protective effect of green tea polyphenol (-)-epigallocatechin gallate and other antioxidants on lipid peroxidation in gerbil brain homogenates

Authors
Seong-Ryong LeeKi-Jo ImSeong-Il SuhJung-Gil Jung
Department
Dept. of Pharmacology (약리학); Dept. of Microbiology (미생물학); Dept. of Anesthesiology & Pain Medicine (마취통증의학); Brain Research Institute (뇌연구소)
Issue Date
2003
Citation
Phytotherapy Research, Vol.17(3) : 206-209, 2003
ISSN
0951-418X
Abstract
The aim of this study was to compare the protective effects of green tea polyphenol (-)-epigallocatechin gallate (EGCG) and other well-known antioxidants on the lipid peroxidation in gerbil brain homogenates. Oxidative stress was induced by H2O2 (10 mM) or ferrous ammonium sulfate (5 μM) and lipid peroxidation was studied. Hydrogen peroxide and ferrous ions are capable of oxidizing a wide range of substrates and causing biological damage. The reaction, referred to as the Fenton process, is complex and can generate both hydroxyl radicals and higher oxidation states of the iron. Thiobarbituric acid-reactive substances (TBA-RS) were used as a marker of lipid peroxidation. EGCG, trolox, lipoic acid, and melatonin reduced H2O2- or ferrous ion-induced lipid peroxidation in a concentration-dependant manner. In reducing the H2O2-induced lipid peroxidation, IC50 values of antioxidants were as follows: EGCG (0.66 μM), trolox (37.08 μM), lipoic acid (7.88 mM), and melatonin (19.11 mM). In reducing the ferrous ion-induced lipid peroxidation, IC50 values of antioxidants were as follows: EGCG (3.32 μM), trolox (75.65 μM), lipoic acid (7.63 mM), and melatonin (15.48 mM). Under the in vitro conditions of this experiment, EGCG was the most potent antioxidant in inhibiting H2O2 or ferrous ion-induced lipid peroxidation in the gerbil brain homogenates. Copyright © 2003 John Wiley & Sons, Ltd. Keywords: EGCG; trolox; lipoic acid; melatonin; brain homogenate; antioxidant; lipid peroxidation.
Keywords
EGCGtroloxlipoic acidmelatoninbrain homogenateantioxidantlipid peroxidation
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/33669
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Anesthesiology & Pain Medicine (마취통증의학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pharmacology (약리학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Brain Research Institute (뇌연구소)
Keimyung Author(s)
이성용; 서성일; 정정길
Full Text
https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.1090
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