A new strategy for the diagnosis of MAGE-expressing cancers

Authors
Jong-Wook ParkTaeg Kyu KwonIn-Ho KimSoo-Sang SohnYou-Sah KimChun-Il KimOk Suk BaeKyung Seop LeeKang-Dae LeeCheong-Sam LeeHee-Kyung ChangByung-Kil ChoeSu Yul AhnChang-Ho Jeon
Department
Dept. of Immunology (면역학); Dept. of Surgery (외과학); Dept. of Urology (비뇨의학); Institute for Medical Science (의과학연구소)
Issue Date
2002
Citation
Journal of Immunological Methods, Vol.266(1-2) : 79-86, 2002
ISSN
0022-1759
Abstract
The expression of melanoma antigen gene (MAGE), coding for tumor antigens recognized by cytotoxic T cell, is highly specific to cancer cells, but their use in the detection of a few cancer cells by reverse transcription-polymerase chain reaction (RT-PCR) has been limited by the low frequency of expression of individual MAGE genes. In order to increase MAGE detection rate in RT-PCR assay, here, we designed multiple MAGEs recognizing primers (MMRPs) that can bind to the sequences of cDNA of MAGE-1, -2, -3, -4a, -4b, -5a, -5b and-6 (MAGE 1–6) together. The nested RT-PCR assay using MMRPs, MAGE 1–6 assay, detected MAGE messages of 1 to 5 SNU484 cells in a background of 107 SNU638 cells. MAGE detection rate of MAGE 1–6 assay in cancers was higher than that of nested RT-PCR that detects single MAGE gene expression. The expressions of MAGE genes was detected by MAGE 1–6 assay in 70.4% (19/27) of head and neck cancer tissues, 91.7% (11/12) of breast cancer tissues, 75% (9/12) of lung cancer tissues. However, they were not detected in 18 benign lesions and 20 normal head and neck tissues and 30 blood samples from healthy donor. In conclusions, MAGE 1–6 assay can detect any cancer cells that express at least one of eight MAGE subtype genes, and this method may be very useful for the diagnosis of MAGE-expressing cancers.
Keywords
MAGENested RT-PCRCancer diagnosis
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/33787
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Urology (비뇨의학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
Keimyung Author(s)
박종욱; 권택규; 김인호; 손수상; 김유사; 김천일; 배옥석
Full Text
https://www.sciencedirect.com/science/article/pii/S0022175902001059?via%3Dihub
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