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mRNA Expression and RNA Editing (2451 C-to-U) of IL-12 Receptor β2 in Adult Atopic Patients

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Affiliated Author(s)
이원목하정숙류남희전동석김재룡
Alternative Author(s)
Lee, Won MokHa, Jung SookRyoo, Nam HeeJeon, Dong SeokKim, Jae Ryong
Journal Title
Journal of Korean Medical Science
ISSN
1011-8934
Issued Date
2006
Keyword
interleukin-12 receptorIL-12R 2Gene Expression ProfilingAtopyAsthmaDermatitis, AtopicRNA Editing
Abstract
Interleukin (IL)-12 activates T helper (Th) 1 cells to produce interferon (IFN)-γ which inhibits atopic inflammation. IL-12 acts through interaction with its receptor, especially β2 subunit. In several studies, the low production of IFN-γ in peripheral mononuclear cells of atopic patients on response to IL-12 stimulation has been reported. Therefore we investigated the IL-12 receptor β2 (IL-12Rβ2) mRNA expression and RNA editing, nucleotide 2451 C-to-U conversion, to find the cause of low responsiveness to IL-12 in atopy. Quantitative real time PCR for mRNA expression and sequence analysis for RNA editing were performed in 80 atopic patients and 54 healthy controls. The expression of IL-12Rβ2 mRNA was significantly lower in atopic patients than healthy controls (p<0.05). In sequence analysis, RNA editing on nucleotide 2451 was not found from either atopic patients or healthy controls. In additional evaluation, there was no relationship between expression of IL-12Rβ2 mRNA and serum total IgE or blood eosinophil count. Reduced IL-12Rβ2 mRNA expression in atopic patients indicate the reduced capacity to respond to IL-12 which induce IFN-γ production and this may contribute to Th2-skewed immune response in atopy.
Department
Dept. of Laboratory Medicine (진단검사의학)
Publisher
School of Medicine
Citation
Eun Jin Kim et al. (2006). mRNA Expression and RNA Editing (2451 C-to-U) of IL-12 Receptor β2 in Adult Atopic Patients. Journal of Korean Medical Science, 21(6), 1070–1074. doi: 10.3346/jkms.2006.21.6.1070
Type
Article
ISSN
1011-8934
DOI
10.3346/jkms.2006.21.6.1070
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33851
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
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