계명대학교 의학도서관 Repository

Protective effect of melatonin on TNF-α-induced muscle atrophy in L6 myotubes

Metadata Downloads
Affiliated Author(s)
박재형임승순송대규임정근
Alternative Author(s)
Park, Jae HyungIm, Seung SoonSong, Dae KyuLim, Jeong Geun
Journal Title
Journal of Pineal Research
ISSN
0742-3098
Issued Date
2013
Keyword
antioxidant enzyme activityL6 myotubemelatoninmuscle atrophymuscle cell deathreactive oxygen speciesTNF-α
Abstract
Muscle atrophy, characterized by decreased cell number and size, is a serious concern for patients afflicted with inflammatory diseases. Mounting evidence indicates that tumor necrosis factor alpha (TNF-α) plays a critical role in muscle atrophy in a number of clinical settings. We hypothesize that reactive oxygen species (ROS) mediate TNF-α-induced muscle cell death and hypotrophy. Recently, melatonin has attracted attention because of its free-radical scavenging and antioxidant properties. The aim of the current study was to evaluate the possible protective role of melatonin in TNF-α-induced muscle cell death and hypotrophy in rat L6 myotubes. To examine this possible role, L6 myotubes were exposed to various concentrations of recombinant TNF-α for 24 hr. We found that TNF-α at a concentration of 100 ng/mL induced ROS generation and decreased cell viability. Further analysis revealed that apoptosis, but not autophagy, may be important for TNF-α-induced cell death. Melatonin significantly attenuated TNF-α-induced ROS generation and apoptosis. In addition, decreased muscle fiber diameter and increased muscle cell proteolysis by TNF-α was highly attenuated by treatment with melatonin. The effects of melatonin were mediated neither through its plasmalemmal receptors nor by modulating the nuclear factor kappa B pathway activated by TNF-α. Taken together, these results suggest that TNF-α may mediate ROS-induced muscle cell death and hypotrophy and that melatonin may be a useful tool for protecting against muscle atrophy stemming from inflammatory diseases.
Department
Dept. of Physiology (생리학)
Dept. of Neurology (신경과학)
Publisher
School of Medicine
Citation
Jae-Hyung Park et al. (2013). Protective effect of melatonin on TNF-α-induced muscle atrophy in L6 myotubes. Journal of Pineal Research, 54(4), 417–425. doi: 10.1111/jpi.12036
Type
Article
ISSN
0742-3098
DOI
10.1111/jpi.12036
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33880
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Neurology (신경과학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.