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Isolation and Identification of Phlorotannins from Ecklonia stolonifera with Antioxidant and Anti-inflammatory Properties

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Affiliated Author(s)
장병철
Alternative Author(s)
Jang, Byeong Churl
Journal Title
Journal of agricultural and food chemistry
ISSN
0021-8561
Issued Date
2009
Keyword
Ecklonia stoloniferaantioxidantanti-inflammatoryphlorotanninlipopolysaccharideRAW 264.7 cells
Abstract
Bioactivity-guided fractionation of Ecklonia stolonifera was used to determine the chemical identity of bioactive constituents, with potent antioxidant activities. The structures of the phlorotannins were determined on the basis of spectroscopic analysis, including NMR and mass spectrometry analysis. The antioxidant activities of the isolated compounds were evaluated by free radical scavenging activities in both in vitro and cellular systems. The anti-inflammatory effects of the isolated compounds were evaluated by determining their inhibitory effects on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophage cells. The results indicated that phlorofucofuroeckol A, dieckol, and dioxinodehydroeckol showed potential radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl. Among them, phlorofucofuroeckol A and dieckol significantly suppressed the intracellular reactive oxygen species level assayed by 2′,7′-dichlorofluorescein diacetate assay in LPS-induced RAW 264.7 cells. Phlorofucofuroeckol A significantly inhibited the LPS-induced production of NO and PGE2 through the down-regulation of inducible nitric oxide synthase and cyclooxygenase 2 protein expressions. In conclusion, these results suggest that phlorofucofuroeckol A has a potential for functional foods with antioxidant and anti-inflammatory activities.
Department
Dept. of Molecular Medicine (분자의학)
Publisher
School of Medicine
Citation
A-REUM KIM et al. (2009). Isolation and Identification of Phlorotannins from Ecklonia stolonifera with Antioxidant and Anti-inflammatory Properties. Journal of agricultural and food chemistry, 57(9), 3483–3489. doi: 10.1021/jf900820x
Type
Article
ISSN
0021-8561
DOI
10.1021/jf900820x
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33941
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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