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All-trans-retinoic acid down-regulates elastin promoter activity elevated by ultraviolet B irradiation in cultured skin fibroblasts

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Author(s)
이규석류영욱김병천
Alternative Author(s)
Lee, Kyu SukRyoo, Young WookKim, Byung Chun
Publication Year
1998
Keyword
Retinoic acidUVBElastin
Abstract
Topical tretinoin therapy produces clinical improvements in the fine wrinkling of photodamaged skin, possibly by enhancement of collagen synthesis. A major biochemically and histologically detectable change in photodamaged skin is the accumulation of abnormal elastic fibers (elastotic material). However, little is known about the effects of retinoic acid and ultraviolet B (UVB) on elastin gene expression. Consequently, we examined the effects of all-trans-retinoic acid (t-RA) and UVB on elastin gene expression in cultured human skin fibroblasts in vitro. Elastin mRNA gene expression was up-regulated in response to UVB by ≈3-fold, in a dose dependent manner, between 3 and 10 mJ/cm2 doses. Similar results were obtained by chloramphenicol acetyltransferase assay, in which a maximal promoter activation more than 5.4-fold that in nonirradiated controls occurred after a single dose of 20 mJ/cm2. Also, t-RA inhibited the increase in elastin mRNA level following a single exposure to UVB by approximately 16%, and the increase in promotor activity by about 65%. The inhibitory effect of t-RA on elastin induced by UVB was also demonstrated by indirect immunofluorescence studies. Taken together, t-RA down-regulated human elastin gene expression elevated by a single exposure to UVB at transcriptional and possibly protein levels. These results suggest that the anti-photoaging effect of t-RA may be related, at least in part, to down-regulation of elastin gene expression elevated by UVB.
Department
Dept. of Dermatology (피부과학)
Publisher
School of Medicine
Citation
Journal of Dermatological Science, Vol.17(3) : 182-189, 1998
Type
Article
ISSN
0923-1811
DOI
10.1016/S0923-1811(98)00004-8
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34015
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