Identification of CD44 splice variant in Korean colorectal cancers and cell lines

Authors
Seong-Il SuhWon-Ki BaekJong-Wook ParkOk-Suk BaeMin-Ho SuhByung-Kil Choe
Department
Dept. of Microbiology (미생물학); Dept. of Immunology (면역학); Dept. of Surgery (외과학); Institute for Medical Science (의과학연구소)
Issue Date
1995
Citation
Journal of Korean Medical Science, Vol.10(3) : 169-175, 1995
ISSN
1011-8934
Abstract
CD44 is a glycoprotein expressed in a wide variety of cell types. Recently expression of some alternatively-spliced variants of CD44 transcripts (CD44v) has been suggested to play a potential role in tumor metastasis and the detection of CD44v containing exon 6 to 11 may be helpful for the diagnosis of cancers. Expressions of CD44v containing exon 6 to 11 were investigated in 20 human colorectal cancer samples, peripheral blood leukocytes isolated from colorectal cancer patients, and 4 colorectal cancer cell lines using reverse transcription-polymerase chain reaction and Southern blot analysis. The standard form of CD44 transcripts was expressed in all samples tested. CD44v containing exon 6 to 11 was expressed in 18 cases of colorectal cancers (sensitivity = 90%), 3 out of 4 cell lines, and one normal tissue (specificity = 95%). These results suggest that the expression of CD44v containing exon 6 to 11 can be regarded as tumor specific and that this marker may be helpful for the early diagnosis of colon cancers, if specimens from the early stage are available.
Keywords
CD44 splice variantColorectal cancer
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34079
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
1. Journal Papers (연구논문) > 3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
Keimyung Author(s)
서성일; 백원기; 박종욱; 배옥석; 서민호; 최병길
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