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DNA-mediated immunization of mice with plasmid encoding HBs antigen

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Affiliated Author(s)
박종욱최병길서민호
Alternative Author(s)
Park, Jong WookChoe, Byung KilSuh, Min Ho
Journal Title
Journal of Korean Medical Science
ISSN
1011-8934
Issued Date
1999
Keyword
VaccinesDNAHepatitis B surface antigens
Abstract
In order to develop an experimental DNA vaccine for the prevention and treatment of hepatitis B virus infection, hepatitis B virus surface antigen (HBsAg) DNA was subcloned into an E. coli-eukaryotic cell shuttle vector and was expressed in the Baculovirus expression system. Intramuscular, intradermal, and intraperitoneal injections of 30 microg of the plasmid DNA expressing HBsAg induced humoral and cellular immune responses in ICR mice. The first IgG antibodies were detected after ten days and specific IgG antibody titers peaked after two months of a single intramuscular DNA injection. Anti-HBs antibody titers gradually increased and peaked at four months following intradermal DNA injection, and in case of intraperitoneal injection they peaked at seven months. Generation of HBs-specific helper T lymphocytes was also investigated through the production of interleukin-2 by T helper cells. Boosting effects of HBs DNA were investigated without much results. In general, DNA-mediated HBs immunization induced humoral and cellular immune responses in mice that appears to simulate immune responses in human during the course of HBV vaccination.
Department
Dept. of Immunology (면역학)
Dept. of Microbiology (미생물학)
Institute for Medical Science (의과학연구소)
Publisher
School of Medicine
Citation
Soo-Jung Yoon et al. (1999). DNA-mediated immunization of mice with plasmid encoding HBs antigen. Journal of Korean Medical Science, 14(2), 187–192. doi: 10.3346/jkms.1999.14.2.187
Type
Article
ISSN
1011-8934
DOI
10.3346/jkms.1999.14.2.187
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34104
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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